Prof. Andrea Mattevi

Andrea Mattevi studied biology. He received the PhD at the Department of Biophysical Chemistry, University of Groningen, The Netherlands under the supervision of Prof. Dr. W.G.J. Hol. He was then post-doctoral EMBO-fellow at the MRC Laboratory of Molecular Biology, Cambridge, UK in the group of J. Walker and A.G.W. Leslie. He is presently full professor in Molecular at the Biology Department of Biology and Biotechnology, University of Pavia, Italy

Key expertise

The general research theme of the structural – molecular biology group headed by Dr. Andrea Mattevi at the Dept. of Biology and Biotechnology of the University of Pavia is the structural, evolutionary, and chemical basis of enzyme catalysis and its mechanisms. Their work is addressing the investigation of medically relevant enzymes with interesting mechanistic properties, such as complex multifunctional systems, proteins performing unusual catalytic functions, and, more recently, intact metabolons encompassing entire metabolic pathways. The research integrates biochemical, structural, and computational methods to investigate protein- and enzyme-based molecular mechanisms, with the goal of leveraging this knowledge for therapeutic development. The laboratory has been continuously funded by national and international grants (EU, ERC, NIH).

The laboratory is equipped with several fermenters for bacterial cell growth, dedicated rooms for large scale mammalian cell culture, cell disruptors, five AKTA systems for protein purification, spectrophotometers and fluorimeters for enzymatic assays, Shimadzu HPLC with a fluorescence detector for pre-purification stability screening of proteins, plate reader for medium-throughput assays, multi-angle light scattering detector for the analysis of protein homogeneity, a system for the label-free evaluation of protein thermal stability, and an instrument for microscale thermophoresis to probe protein binding. Centre of Large Equipment (CGS) of the University of Pavia provided regular access to cryoEM, mass spectrometry, gas-chromatography, and HPLC for analytical chemistry that are needed for enzymatic product analysis.

Hosting Institution

The University of Pavia (Università di Pavia) is one of the oldest universities in the world, founded in 1361. Rich in history and tradition, it has played a significant role in European academic life for centuries. Originally established as a Studium Generale by the Holy Roman Empire, it has since developed into a modern and prestigious institution, known for its academic excellence and interdisciplinary research. The university offers a wide range of undergraduate, graduate, and doctoral programs across 18 departments, covering fields such as medicine, engineering, law, economics, humanities, and natural sciences. It is particularly renowned for its medical and scientific faculties, as well as for engineering and international law. Pavia’s strong focus on research is supported by multiple specialized centers and collaborations with institutions around the world. The university is also a member of international academic networks like the Coimbra Group. Beyond academics, the University of Pavia is distinguished by its unique collegiate system. It hosts a network of historic university colleges that provide not only accommodation but also cultural, social, and academic enrichment. The university’s campus is embedded in the picturesque town of Pavia, known for its medieval charm and vibrant student life, just 35 kilometers south of Milan.

Contact

andrea.mattevi@unipv.it

University of Pavia
Department of Biology and Biotechnology
Via Ferrata 9
27100 Pavia
Italy

http://www-9.unipv.it/biocry/

https://twitter.com/@MatteviLab

@mattevilab.bsky.social

https://www.scopus.com/authid/detail.uri?authorId=7005232911&origin=resultslist

https://orcid.org/0000-0002-9523-7128

Relevant Publications

Guerriere, T. B., Vancheri, A., Ricotti, I., Serapian, S. A., Eggerichs, D., Tischler, D., Colombo, G., Mascotti, M. L., Fraaije, M. W., & Mattevi, A. (2025). Dehydrogenase versus oxidase function: the interplay between substrate binding and flavin microenvironment. ACS catalysis, 15(2), 1046–1060. https://doi.org/10.1021/acscatal.4c05944

Nicoll, C. R., Alvigini, L., Gottinger, A., Cecchini, D., Mannucci, B., Corana, F., Mascotti, M. L., & Mattevi, A. (2024). In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis. Nature catalysis, 7(2), 148–160. https://doi.org/10.1038/s41929-023-01087-z

Reis, J., Gorgulla, C., Massari, M., Marchese, S., Valente, S., Noce, B., Basile, L., Törner, R., Cox, H., 3rd, Viennet, T., Yang, M. H., Ronan, M. M., Rees, M. G., Roth, J. A., Capasso, L., Nebbioso, A., Altucci, L., Mai, A., Arthanari, H., & Mattevi, A. (2023). Targeting ROS production through inhibition of NADPH oxidases. Nature chemical biology, 19(12), 1540–1550. https://doi.org/10.1038/s41589-023-01457-5